EFFECT OF OG-1 CARDIOACTIVE PRINCIPLE FROM CHLOROFORM EXTRACT OF Ocimum gratissimum (Linn.) LEAVES ON ISCHAEMIA AND REPERFUSION-INDUCED MYOCARDIAL INJURY

Objective:The present study has been designed to investigate efficacy Of OG-1 cardioactive principle from chloroform extract of Ocimum gratissimum (Linn.) Leaves on ischaemia and reperfusion-Induced myocardial injury. Methods: Various extracts of O. gratissimum (Linn.) leaves were prepared viz. Pet.ether, chloroform, acetone and methanol. Among all the extracts chloroform extract reduce myocardial injury. The chloroform extract further purified using column chromatographic technique that results four major fractions viz F1, F2, F3 and F4. Among these fractions F4 was found to be hot fraction. OG1 (active principle) was isolated from the F4 using column chromatography technique and evaluated for cardioprotective effect. Results: The present study demonstrated that Chloroform extract of O.gratissimum (Linn.) leaves and further fraction F4 of chloroform extract significantly prevented myocardial infarct size as compared with that of standard ramipril. OG1 (active principle) was isolated from fraction F4 of chloroform extract showing cardioprotective effect at dose level of 50mg/kg body weight as compared with that of standard ramipril (1mg/kg body weight). Conclusion:These results suggest that OG1 is active cardioprotective principle of O. gratissimum (Linn.) chloroform leaves extract.